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KMID : 0861020180330060001
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Korea Journal of Herbology
2018 Volume.33 No. 6 p.1 ~ p.8
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Anti-oxidant effect of ethanol extract from mixture including Caryophylli Flos,Aucklandiae Radix and Angelicae Dahuricae Radix
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Youn Seok-Na
Kim Yoo-Jin
Kim Mi-Ryeo
Yoo Wang-Keun
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Abstract
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Objectives : Herbal medicinal mixture (JMB) are consisted of Caryophylli Flos, Aucklandiae Radix and Angelicae Dahuricae Radix. Each of herbal medicines has studied on anti-oxidant effect. So this study was conducted to investigate efficacy and potency of JMB on anti-oxidation.
Methods : The JMB was extracted at room temperature by 80% ethanol. And total polyphenol contents, total flavonoid contents in JMB ethanol extract were determined by colorimetric method. Also, DPPH, ABTS free radical scavenging capacity and reducing power of JMB ethanol extract were measured at 100, 500, 1000, 5000 ㎍/㎖ concentrations by spectrometric assay. Positive control was used BHA (butylated hydroxyanisole).
Results : The total polyphenol contents and total flavonoid contents of the extract were 55.38 ㎎/TAEg, 513.72 ㎎/RUEg, respectively. Also, DPPH free radical scavenging capacity and reducing power of JMB ethanol extract in treated concentrations (100, 500, 1000, 5000 ㎍/㎖) increased dose dependently. In particular, DPPH free radical scavenging capacity of JMB ethanol extract at 500, 1000, 5000 ㎍/㎖ was similar to positive control (BHA) at high concentration (50, 100 ㎍/㎖). ABTS free radical scavenging capacity of JMB ethanol extract at 500, 1000, 5000 ug/ml was similar to BHA at high concentration (50, 100 ㎍/㎖). Also, reducing power was showed that JMB ethanol extract at 5000 ㎍/㎖ was similar to BHA at high concentration (50, 100 ㎍/㎖).
Conclusions : These results suggest that JMB ethanol extract has effects to scavenge free radicals. Therefore, JMB has potential and applicable benefits for development of materials and products to have anti-oxidation functions.
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KEYWORD
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Anti-oxidant, JMB, free radical scavenging capacity, total polyphenols, total flavonoids
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